Journal: Scientific Reports

Article Title: Rational design of synthetic antimicrobial peptides based on the Escherichia coli ShoB toxin

doi: 10.1038/s41598-025-98330-3

Figure Lengend Snippet: MIC and MBC bacterial panel assessment of peptide 1 variants*.

Article Snippet: Bacterial susceptibility was next assessed by adding an equal volume of E. coli ATCC 25922 inoculum to the wells and following the protocol of the above described MIC assay.

Techniques: Sequencing

Journal: Scientific Reports

Article Title: Rational design of synthetic antimicrobial peptides based on the Escherichia coli ShoB toxin

doi: 10.1038/s41598-025-98330-3

Figure Lengend Snippet: Effect of plasmid-based expression of native and truncated variants of the ShoB peptide. Bar plot showing the percentage of surviving Escherichia coli cells (y-axis) after expression of the native, N-terminally truncated or C-terminally truncated variants of the ShoB peptide. The peptides were expressed intracellularly from a plasmid (pET28b(+)) encoding native and truncated variants (see Materials and Methods for details). Standard deviations are indicated in the plot. The experiment was performed in triplicate.

Article Snippet: Bacterial susceptibility was next assessed by adding an equal volume of E. coli ATCC 25922 inoculum to the wells and following the protocol of the above described MIC assay.

Techniques: Plasmid Preparation, Expressing

Journal: Scientific Reports

Article Title: Rational design of synthetic antimicrobial peptides based on the Escherichia coli ShoB toxin

doi: 10.1038/s41598-025-98330-3

Figure Lengend Snippet: Time-kill kinetics assessment of best peptide hits. Graphs showing the colony-forming units per mL (CFU/mL; y-axis) of E. coli ATCC 25922 after incubation with peptide 1a ( a ), 1a-N10 ( b ), 1a-N9 ( c ), and 1a-N5 ( d ) for 0, 0.25, 0.5, 2, 5 and 24 h (x-axis). The peptide concentrations used are indicated by color coding of the respective graphs, while standard deviations are shown by shaded areas. The limit of bacterial detection in this assay was 100 CFU/mL (see Materials and Methods).

Article Snippet: Bacterial susceptibility was next assessed by adding an equal volume of E. coli ATCC 25922 inoculum to the wells and following the protocol of the above described MIC assay.

Techniques: Incubation

Journal: Scientific Reports

Article Title: Rational design of synthetic antimicrobial peptides based on the Escherichia coli ShoB toxin

doi: 10.1038/s41598-025-98330-3

Figure Lengend Snippet: Assessment of MIC and MBC of D-form variants (of 1a and 1a-N10-D) in a panel of bacteria*.

Article Snippet: Bacterial susceptibility was next assessed by adding an equal volume of E. coli ATCC 25922 inoculum to the wells and following the protocol of the above described MIC assay.

Techniques: Bacteria, Sequencing

Journal: Scientific Reports

Article Title: Rational design of synthetic antimicrobial peptides based on the Escherichia coli ShoB toxin

doi: 10.1038/s41598-025-98330-3

Figure Lengend Snippet: Effect of protease treatment on antibacterial activity of L- and D-form peptides. Bar plots showing the effect of a pre-incubation step, in which peptides 1a , D-1a , 1a-N10 or D-1a-N10 were treated with 0.125 mg/mL trypsin for 1 h ( a , c ) or 6 h ( b , d ), prior to testing in a growth inhibition assay with E. coli ATCC 25922. The optical density (OD; y-axis) of the E. coli cultures was assessed after exposure for 20–23 h to trypsin-treated peptides at the indicated concentrations (x-axis). The experiment was performed in triplicate, and standard deviations are indicated in the plots.

Article Snippet: Bacterial susceptibility was next assessed by adding an equal volume of E. coli ATCC 25922 inoculum to the wells and following the protocol of the above described MIC assay.

Techniques: Activity Assay, Incubation, Growth Inhibition Assay

Journal: Scientific Reports

Article Title: Rational design of synthetic antimicrobial peptides based on the Escherichia coli ShoB toxin

doi: 10.1038/s41598-025-98330-3

Figure Lengend Snippet: Frequency of resistance (FoR) after treatment with L- and D-form peptide hits for 24 or 48 h. Bar plots showing the mutation rate per cell per generation of E. coli ATCC 25922 treated with 4 × MIC of colistin, peptide 1a , D-1a , 1a-N10 or D-1a-N10 for 24 or 48 h. The values were calculated by using the p 0 method (see Materials & Methods for details). The experiments were performed in triplicate, and standard errors of the mean are indicated in the plots.

Article Snippet: Bacterial susceptibility was next assessed by adding an equal volume of E. coli ATCC 25922 inoculum to the wells and following the protocol of the above described MIC assay.

Techniques: Mutagenesis